Special Seminar with Hiroki Ueda, MD, PhD … procedure for producing knock-out (KO) mice by ‘Triple-CRISPR’ method that targets a single gene by triple gRNAs in the CRISPR/Cas9 system achieves almost perfect KO efficiency (96%-100%). …
The unexpected precision of an inducible transcription factor … neuron. Using ex vivo electrophysiology, optogenetics, and CRISPR/Cas9 manipulations of Npas4, we find NPAS4 selectively …